Multiplexing RMCE: versatile extensions of the Flp-recombinase-mediated cassette-exchange technology.

@article{Turan2010MultiplexingRV,
  title={Multiplexing RMCE: versatile extensions of the Flp-recombinase-mediated cassette-exchange technology.},
  author={Soeren Turan and Johannes Kuehle and Axel Schambach and Christopher Baum and Juergen Bode},
  journal={Journal of molecular biology},
  year={2010},
  volume={402 1},
  pages={52-69}
}
There are strong indications, but as yet no proof, that extended 48-bp Flp recombinase targets (FRTs) represent unique targets in all eukaryotic genomes investigated, and that recombinase-mediated cassette exchange is not hampered by the occurrence of genomic pseudo sites. This encouraged the present study in which we explore the feasibility of exchanging, in a given cell, two distinct genomically anchored cassettes, each flanked by a unique set of two heterospecific FRT sites. Mutant FRTs have… CONTINUE READING
24 Citations
37 References
Similar Papers

Citations

Publications citing this paper.
Showing 1-10 of 24 extracted citations

References

Publications referenced by this paper.
Showing 1-10 of 37 references

Retroviral polyproteinmediated transduction of Flp recombinase

  • C. Voelkel, M. Galla, T. Maetzig, E. Warlich, J. Kuehle, D Zychlinski
  • Proc. Natl Acad
  • 2010

Similar Papers

Loading similar papers…