Multiplexed quantification of proteins and transcripts in single cells

  title={Multiplexed quantification of proteins and transcripts in single cells},
  author={V. M. Peterson and K. Zhang and N. Kumar and J. Wong and L. Li and Douglas C Wilson and R. Moore and T. Mcclanahan and S. Sadekova and J. Klappenbach},
  journal={Nature Biotechnology},
  • V. M. Peterson, K. Zhang, +7 authors J. Klappenbach
  • Published 2017
  • Biology, Medicine
  • Nature Biotechnology
  • We present a tool to measure gene and protein expression levels in single cells with DNA-labeled antibodies and droplet microfluidics. Using the RNA expression and protein sequencing assay (REAP-seq), we quantified proteins with 82 barcoded antibodies and >20,000 genes in a single workflow. We used REAP-seq to assess the costimulatory effects of a CD27 agonist on human CD8+ lymphocytes and to identify and characterize an unknown cell type. 

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