Multiplexed quantification of proteins and transcripts in single cells

@article{Peterson2017MultiplexedQO,
  title={Multiplexed quantification of proteins and transcripts in single cells},
  author={Vanessa M. Peterson and Kelvin Xi Zhang and Namit Kumar and Jerelyn Wong and Lixia Li and Douglas C. Wilson and Renee Moore and Terrill K. Mcclanahan and Svetlana Sadekova and Joel A. Klappenbach},
  journal={Nature Biotechnology},
  year={2017},
  volume={35},
  pages={936-939}
}
We present a tool to measure gene and protein expression levels in single cells with DNA-labeled antibodies and droplet microfluidics. Using the RNA expression and protein sequencing assay (REAP-seq), we quantified proteins with 82 barcoded antibodies and >20,000 genes in a single workflow. We used REAP-seq to assess the costimulatory effects of a CD27 agonist on human CD8+ lymphocytes and to identify and characterize an unknown cell type. 

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A simple and reliable method for conjugation of oligonucleotides with antibodies and a protocol for their use in single-cell transcriptome sequencing is presented.

Highly multiplexed single-cell RNA-seq by DNA oligonucleotide tagging of cellular proteins

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Combined aptamer and transcriptome sequencing of single cells

This work demonstrates a method to simultaneously characterize the transcriptomes and proteomes of single cells at high throughput using aptamer probes and droplet-based single cell sequencing, and distinguishes distinct cell types based on aptamer surface binding and gene expression patterns.

Combined Measurement of RNA and Protein Expression on a Single-Cell Level.

The BD Rhapsody single-cell analysis system protocol for 3' mRNA whole transcriptome analysis (WTA), combined with AbO- and Sample Tag library preparation is described, overcoming the limit of using few surface markers as occurs in flow cytometry.

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