Multiplexed quantification of proteins and transcripts in single cells

  title={Multiplexed quantification of proteins and transcripts in single cells},
  author={Vanessa M. Peterson and Kelvin Xi Zhang and Namit Kumar and Jerelyn Wong and Lixia Li and Douglas C. Wilson and Renee Moore and Terrill K. Mcclanahan and Svetlana Sadekova and Joel A. Klappenbach},
  journal={Nature Biotechnology},
We present a tool to measure gene and protein expression levels in single cells with DNA-labeled antibodies and droplet microfluidics. Using the RNA expression and protein sequencing assay (REAP-seq), we quantified proteins with 82 barcoded antibodies and >20,000 genes in a single workflow. We used REAP-seq to assess the costimulatory effects of a CD27 agonist on human CD8+ lymphocytes and to identify and characterize an unknown cell type. 

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