Multiplexed protein detection using antibody-conjugated microbead arrays in a microfabricated electrophoretic device.

  title={Multiplexed protein detection using antibody-conjugated microbead arrays in a microfabricated electrophoretic device.},
  author={Kristopher David Barbee and Alexander P. Hsiao and Eric E. Roller and Xiaohua Huang},
  journal={Lab on a chip},
  volume={10 22},
We report the development of a microfabricated electrophoretic device for assembling high-density arrays of antibody-conjugated microbeads for chip-based protein detection. The device consists of a flow cell formed between a gold-coated silicon chip with an array of microwells etched in a silicon dioxide film and a glass coverslip with a series of thin gold counter electrode lines. We have demonstrated that 0.4 and 1 μm beads conjugated with antibodies can be rapidly assembled into the… 

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