Multiplexed protein-DNA cross-linking: Scrunching in transcription start site selection

@article{Winkelman2016MultiplexedPC,
  title={Multiplexed protein-DNA cross-linking: Scrunching in transcription start site selection},
  author={Jared T. Winkelman and Irina O. Vvedenskaya and Yuanchao Zhang and Yu Zhang and Jeremy G. Bird and Deanne Taylor and Richard L Gourse and Richard H Ebright and Bryce E. Nickels},
  journal={Science},
  year={2016},
  volume={351},
  pages={1090-1093}
}
In bacterial transcription initiation, RNA polymerase (RNAP) selects a transcription start site (TSS) at variable distances downstream of core promoter elements. Using next-generation sequencing and unnatural amino acid–mediated protein-DNA cross-linking, we have determined, for a library of 410 promoter sequences, the TSS, the RNAP leading-edge position, and the RNAP trailing-edge position. We find that a promoter element upstream of the TSS, the “discriminator,” participates in TSS selection… CONTINUE READING
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