Multiplexed electrical detection of cancer markers with nanowire sensor arrays

  title={Multiplexed electrical detection of cancer markers with nanowire sensor arrays},
  author={Gengfeng Zheng and Fernando Patolsky and Yi Cui and Wayne U. Wang and Charles M. Lieber},
  journal={Nature Biotechnology},
We describe highly sensitive, label-free, multiplexed electrical detection of cancer markers using silicon-nanowire field-effect devices in which distinct nanowires and surface receptors are incorporated into arrays. [] Key Result Protein markers were routinely detected at femtomolar concentrations with high selectivity, and simultaneous incorporation of control nanowires enabled discrimination against false positives.
Ultrasensitive Detection of Dual Cancer Biomarkers with Integrated CMOS-Compatible Nanowire Arrays.
A two-channel PDMS microfluidic integrated CMOS-compatible silicon nanowire (SiNW) field-effect transistor arrays with potentially single use for label-free and ultrasensitive electrical detection of cancer biomarkers and opens up great opportunities for a point-of-care test (POCT) for quick screening and early diagnosis of cancer and other complex diseases.
Top-Down Fabricated Silicon Nanowire Arrays for Field-Effect Detection of Prostate-Specific Antigen
The electrical approach using the Si NW-ISFET platform shows a lower limit of detection and a wide dynamic range of the assay, and should be utilized to detect multiple biomarkers in one assay to obtain more reliable information about cancer-related diseases.
Direct ultrasensitive electrical detection of prostate cancer biomarkers with CMOS-compatible n- and p-type silicon nanowire sensor arrays.
The highly selective, simultaneous and multiplexed detection of PSA marker at attomolar concentrations, a level useful for clinical diagnosis of prostate cancer, was demonstrated and the detection ability was corroborated to be effective by comparing the detection results at different pH values.
Label-Free Multiplexed Electrical Detection of Cancer Markers on a Microchip Featuring an Integrated Fluidic Diode Nanopore Array.
An integrated array of glass nanopores on a silicon microchip fabricated in a batch process through low-resolution photolithography and standard semiconductor processing tools is introduced and ultrasensitive, label-free, multiplexed electrical detection of cancer-marker proteins in real time through charge-dependent ionic current rectification is demonstrated.
Silicon nanowire biosensor for highly sensitive and multiplexed detection of oral squamous cell carcinoma biomarkers in saliva.
A multiplexed detection methodology for IL-8 and TNF-α detection in saliva using SiNW FET biosensors is developed and is expected to be widely used for the early diagnosis of oral squamous cell carcinoma.
Nanotube-antibody biosensor arrays for the detection of circulating breast cancer cells.
A nanoscale oncometer with single cell sensitivity with a diameter 1000 times smaller than a cancer cell that functions in a drop of fresh blood is reported, it appears that the binding of a single cell to a single nanotube field effect transistor produced the change in electrical conductivity.


Label-free detection of small-molecule-protein interactions by using nanowire nanosensors.
It is demonstrated that the silicon nanowire devices can readily and rapidly distinguish the affinities of distinct small-molecule inhibitors and, thus, could serve as a technology platform for drug discovery.
Nanowire Nanosensors for Highly Sensitive and Selective Detection of Biological and Chemical Species
The small size and capability of these semiconductor nanowires for sensitive, label-free, real-time detection of a wide range of chemical and biological species could be exploited in array-based screening and in vivo diagnostics.
Bioassay of prostate-specific antigen (PSA) using microcantilevers
It is reported here that microcantilevers of different geometries have been used to detect two forms of prostate-specific antigen over a wide range of concentrations from 0.2 ng/ml to 60 μg/ml in a background of human serum albumin and human plasminogen at 1 mg/ml, making this a clinically relevant diagnostic technique for prostate cancer.
Electrical detection of single viruses.
Direct, real-time electrical detection of single virus particles with high selectivity by using nanowire field effect transistors is reported, suggesting potential for simultaneous detection of a large number of distinct viral threats at the single virus level.
Nanoparticle-Based Bio-Bar Codes for the Ultrasensitive Detection of Proteins
An ultrasensitive method for detecting protein analytes has been developed and comparable clinically accepted conventional assays for detecting the same target have sensitivity limits of ∼3 picomdar, six orders of magnitude less sensitive than what is observed with this method.
Real-Time, label-free monitoring of tumor antigen and serum antibody interactions.
In vivo cancer targeting and imaging with semiconductor quantum dots
Sensitive and multicolor fluorescence imaging of cancer cells under in vivo conditions are achieved and a whole-body macro-illumination system with wavelength-resolved spectral imaging is integrated for efficient background removal and precise delineation of weak spectral signatures.
Noncovalent functionalization of carbon nanotubes for highly specific electronic biosensors
  • R. Chen, S. Bangsaruntip, H. Dai
  • Biology, Chemistry
    Proceedings of the National Academy of Sciences of the United States of America
  • 2003
An exploration of single-walled carbon nanotubes is presented as a platform for investigating surface–protein and protein–protein binding and developing highly specific electronic biomolecule detectors for detecting clinically important biomolecules such as antibodies associated with human autoimmune diseases.
Supersensitive time-resolved immunofluorometric assay of free prostate-specific antigen with nanoparticle label technology.
The sensitivity of the new assay is equal to that of the ambient-analyte, microspot immunoassay and will be improved by use of optimized, high binding-site density nanoparticle-antibody bioconjugates with reduced nonspecific binding and improved monovalent binding affinity.