Multiplexed assays for detection of mutations in PIK3CA.

  title={Multiplexed assays for detection of mutations in PIK3CA.},
  author={R E Board and Nicola J Thelwell and Paul F Ravetto and Stephen Little and Malcolm Ranson and Caroline Dive and Andrew M Hughes and David Whitcombe},
  journal={Clinical chemistry},
  volume={54 4},
BACKGROUND Mutations in the PIK3CA gene (phosphoinositide-3-kinase, catalytic, alpha polypeptide) have recently been described in a number of cancers, and their detection is currently limited because of the low sensitivity of conventional sequencing techniques. METHODS We combined Amplification Refractory Mutation System (ARMS; AstraZeneca) allele-specific PCR and Scorpions (DxS) to develop assays for tumor-borne PIK3CA mutations and used real-time PCR to develop high-throughput multiplexed… CONTINUE READING

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