Multiplex touchdown PCR for rapid typing of the opportunistic pathogen Propionibacterium acnes.

@article{Barnard2015MultiplexTP,
  title={Multiplex touchdown PCR for rapid typing of the opportunistic pathogen Propionibacterium acnes.},
  author={Emma Barnard and Istv{\'a}n Nagy and Judit Hunyadk{\"u}rti and Sheila Patrick and Andrew McDowell},
  journal={Journal of clinical microbiology},
  year={2015},
  volume={53 4},
  pages={1149-55}
}
The opportunistic human pathogen Propionibacterium acnes is composed of a number of distinct phylogroups, designated types IA1, IA2, IB, IC, II, and III, which vary in their production of putative virulence factors, their inflammatory potential, and their biochemical, aggregative, and morphological characteristics. Although multilocus sequence typing (MLST) currently represents the gold standard for unambiguous phylogroup classification and individual strain identification, it is a labor… CONTINUE READING
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