Multiplex tetra-primer amplification refractory mutation system PCR to detect 6 common germline mutations of the MUTYH gene associated with polyposis and colorectal cancer.

@article{Piccioli2006MultiplexTA,
  title={Multiplex tetra-primer amplification refractory mutation system PCR to detect 6 common germline mutations of the MUTYH gene associated with polyposis and colorectal cancer.},
  author={Patrizia Piccioli and Martina Serra and Viviana Gismondi and Simona Pedemonte and Fabrizio Loiacono and Sonia Lastraioli and Lucio Bertario and Maria De Angioletti and Liliana Varesco and Rosario Notaro},
  journal={Clinical chemistry},
  year={2006},
  volume={52 4},
  pages={739-43}
}
BACKGROUND We describe a simple tetra-primer amplification refractory mutation system PCR (T-ARMS-PCR) for detecting MUTYH mutations, which are associated with colorectal adenomas and colorectal cancer. METHODS We designed specific T-ARMS-PCR assays for 6 mutations (Y165C, G382D, 1395_7delGGA, Y90X, 1103delC, and R231H) selected on the basis of the… CONTINUE READING