Multiplex reverse transcriptase PCR assay for simultaneous detection of three fish viruses.

@article{Williams1999MultiplexRT,
  title={Multiplex reverse transcriptase PCR assay for simultaneous detection of three fish viruses.},
  author={K Williams and Simon Blake and Amy Sweeney and John T Singer and Beth L. Nicholson},
  journal={Journal of clinical microbiology},
  year={1999},
  volume={37 12},
  pages={4139-41}
}
A multiplex reverse transcriptase (RT)-PCR assay was developed for the simultaneous detection of three different fish viruses: infectious pancreatic necrosis virus (IPNV), infectious hematopoietic necrosis virus (IHNV), and viral hemorrhagic septicemia virus (VHSV). The sensitivity levels of the multiplex RT-PCR assay were 100, 1, and 32 50% tissue culture infective doses/ml for IPNV, IHNV, and VHSV, respectively.