Multiplex relative RT-PCR method for verification of differential gene expression.

@article{Spencer1999MultiplexRR,
  title={Multiplex relative RT-PCR method for verification of differential gene expression.},
  author={W E Spencer and Mike J Christensen},
  journal={BioTechniques},
  year={1999},
  volume={27 5},
  pages={
          1044-6, 1048-50, 1052
        }
}
Differential display, suppression subtractive hybridization and other techniques for identification of differentially expressed genes produce fragments of cDNA from mRNAs whose differences in abundance must be verified. This report describes a relative multiplex RT-PCR assay that facilitates the analysis of large numbers of samples for differences in mRNA abundance without the use of radioactivity or blotting. The species of interest is co-amplified with 18S rRNA over a range of cycles followed… CONTINUE READING

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