Multiplex real-time PCR method for detection and quantification of mycotoxigenic fungi belonging to three different genera.

@article{Vegi2013MultiplexRP,
  title={Multiplex real-time PCR method for detection and quantification of mycotoxigenic fungi belonging to three different genera.},
  author={Anuradha Vegi and Charlene E Wolf-Hall},
  journal={Journal of food science},
  year={2013},
  volume={78 1},
  pages={M70-6}
}
UNLABELLED Cereal crop plants are colonized by many fungal species such as Aspergillus ochraceus and Penicillium verrucosum, which produce ochratoxins, and Fusarium graminearum, which produces trichothecene mycotoxins. A multiplex real-time PCR method using TaqMan probes was developed to simultaneously detect and quantify these mycotoxigenic Fusarium, Penicillium and Aspergillus species in cereal grains. Primers and probes used in this method were designed targeting the trichothecene synthase… CONTINUE READING