Multiplex real-time PCR for monitoring Heterobasidion annosum colonization in Norway spruce clones that differ in disease resistance.

@article{Hietala2003MultiplexRP,
  title={Multiplex real-time PCR for monitoring Heterobasidion annosum colonization in Norway spruce clones that differ in disease resistance.},
  author={Ari Mikko Hietala and Morten Eikenes and Harald Kvaalen and Halvor Solheim and Carl Gunnar Fossdal},
  journal={Applied and environmental microbiology},
  year={2003},
  volume={69 8},
  pages={
          4413-20
        }
}
  • Ari Mikko Hietala, Morten Eikenes, +2 authors Carl Gunnar Fossdal
  • Published in
    Applied and environmental…
    2003
  • Biology, Medicine
  • A multiplex real-time PCR assay was developed to monitor the dynamics of the Picea abies-Heterobasidion annosum pathosystem. Tissue cultures and 32-year-old trees with low or high resistance to this pathogen were used as the host material. Probes and primers were based on a laccase gene for the pathogen and a polyubiquitin gene for the host. The real-time PCR procedure was compared to an ergosterol-based quantification method in a tissue culture experiment, and there was a strong correlation… CONTINUE READING

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