Multiplex real-time PCR for exhaustive detection of diarrhoeagenic Escherichia coli.

@article{Hidaka2009MultiplexRP,
  title={Multiplex real-time PCR for exhaustive detection of diarrhoeagenic Escherichia coli.},
  author={Akinori Hidaka and T Hokyo and Kentaro Arikawa and Sami Fujihara and Jun Ogasawara and Atsushi Hase and Yukiko Hara-Kudo and Yoshikazu Nishikawa},
  journal={Journal of applied microbiology},
  year={2009},
  volume={106 2},
  pages={410-20}
}
AIMS The source and routes of diarrhoeagenic Escherichia coli (DEC) have not been clarified because it is difficult to detect these organisms in samples with numerous coliform bacteria. We have developed multiplex real-time PCR assays for exhaustive detection of DEC. METHODS AND RESULTS Primers and TaqMan probes were designed to amplify and quantify one gene (eae, stx1, stx2, elt, est, virB, aggR, astA, and afaB) from each of seven pathotypes of DEC, in duplex or triplex reactions under the… CONTINUE READING

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