Multiplex real-time PCR for detection of an epidemic KPC-producing Klebsiella pneumoniae ST258 clone.

@article{Chen2012MultiplexRP,
  title={Multiplex real-time PCR for detection of an epidemic KPC-producing Klebsiella pneumoniae ST258 clone.},
  author={Liang Chen and Kalyan D. Chavda and Jos{\'e} R. Mediavilla and Yanan Zhao and Henry S. Fraimow and Stephen Graham Jenkins and Michael H. Levi and Tao Hong and Albert D. Rojtman and Christine C Ginocchio and Robert A. Bonomo and Barry Kreiswirth},
  journal={Antimicrobial agents and chemotherapy},
  year={2012},
  volume={56 6},
  pages={
          3444-7
        }
}
We describe a multiplex real-time PCR assay capable of identifying both the epidemic Klebsiella pneumoniae ST258 clone and bla(KPC) carbapenemase genes in a single reaction. The assay displayed excellent sensitivity (100%) and specificity (100%) for identification of ST258 clone and bla(KPC) in a collection of 75 K. pneumoniae isolates comprising 41 sequence types. Our results suggest that this assay is an effective tool for surveillance of this clone among carbapenem-resistant K. pneumoniae… CONTINUE READING

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