Multiplex polymerase chain reaction analysis of UV-A- and UV-B-induced delayed and early mutations in V79 Chinese hamster cells.

@article{Dahle2005MultiplexPC,
  title={Multiplex polymerase chain reaction analysis of UV-A- and UV-B-induced delayed and early mutations in V79 Chinese hamster cells.},
  author={Jostein Dahle and Paul Noordhuis and Trond Stokke and Debbie Hege Svendsrud and Erick P. Kvam},
  journal={Photochemistry and photobiology},
  year={2005},
  volume={81 1},
  pages={
          114-9
        }
}
We previously reported that approximately 10% of V79 Chinese hamster fibroblast populations clonally derived from single cells immediately after irradiation with either ultraviolet B (UV-B, 290-320 nm, mainly 311 nm) or ultraviolet A (UV-A, 320-400 nm, mainly 350-390 nm) radiation exhibit genomic instability. The instability is revealed by relatively high mutation frequencies in the hypoxanthine phosphoribosyl transferase (hprt) gene up to 23 cell generations after irradiation. These delayed… CONTINUE READING
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