Multiplex polymerase chain reaction amplification and differentiation of Entamoeba histolytica and Entamoeba dispar DNA from stool samples.

@article{Nez2001MultiplexPC,
  title={Multiplex polymerase chain reaction amplification and differentiation of Entamoeba histolytica and Entamoeba dispar DNA from stool samples.},
  author={Yurai N{\'u}{\~n}ez and Maria Aparecida Fernandez and David Abner Torres-N{\'u}{\~n}ez and Jessica V L Silva and Iv{\'o}n Montano and Jorge Luis Maestre and L. F. M. Fonte},
  journal={The American journal of tropical medicine and hygiene},
  year={2001},
  volume={64 5-6},
  pages={293-7}
}
Due to the clinical importance of differentiating the two species of the Entamoeba histolytica/Entamoeba dispar complex, we developed a multiplex polymerase chain reaction (PCR) method that overcomes time-consuming and laborious procedures. We report here a DNA extraction protocol using non-fixed stool samples that avoid long lysis-incubation periods through the combined use of zirconium beads and a lysis-supporting buffer. We characterized 49 of 52 stool specimens from Cuban patients with… CONTINUE READING

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