Multiplex pairwise assembly of array-derived DNA oligonucleotides.

  title={Multiplex pairwise assembly of array-derived DNA oligonucleotides.},
  author={Jason C Klein and Marc J. Lajoie and Jerrod J. Schwartz and Eva-Maria Strauch and Jorgen W. Nelson and David P. Baker and Jay Shendure},
  journal={Nucleic acids research},
  volume={44 5},
While the cost of DNA sequencing has dropped by five orders of magnitude in the past decade, DNA synthesis remains expensive for many applications. Although DNA microarrays have decreased the cost of oligonucleotide synthesis, the use of array-synthesized oligos in practice is limited by short synthesis lengths, high synthesis error rates, low yield and the challenges of assembling long constructs from complex pools. Toward addressing these issues, we developed a protocol for multiplex pairwise… CONTINUE READING
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