Multiplex nested RT-PCR for detecting avian influenza virus, infectious bronchitis virus and Newcastle disease virus.

@article{Nguyen2013MultiplexNR,
  title={Multiplex nested RT-PCR for detecting avian influenza virus, infectious bronchitis virus and Newcastle disease virus.},
  author={Thanh Trung Nguyen and Hyuk-Joon Kwon and Il-Hwan Kim and Seung-Min Hong and Won-Jin Seong and Jin-Wook Jang and Jae-hong Kim},
  journal={Journal of virological methods},
  year={2013},
  volume={188 1-2},
  pages={41-6}
}
In this study, multiplex nested RT-PCR (mnRT-PCR) was applied to simultaneous detect multiplex PCR with the higher sensitivity of nested PCR that is required for avian influenza, infectious bronchitis and Newcastle disease virus using two steps of amplification. For the first PCR, primers that were specific for each virus were newly designed from the nucleoprotein gene of AIV, the nucleocapsid protein gene of IBV and the fusion protein gene of NDV to amplify products of 665, 386 and 236… CONTINUE READING