Multiplex messenger assay: simultaneous, quantitative measurement of expression of many genes in the context of T cell activation.

@article{Bernard1996MultiplexMA,
  title={Multiplex messenger assay: simultaneous, quantitative measurement of expression of many genes in the context of T cell activation.},
  author={Karine Bernard and Nathalie Auphan and Samuel Granjeaud and Geneviève Victorero and Anne‐Marie Schmitt‐Verhulst and Bertrand R. Jordan and Catherine Nguyen},
  journal={Nucleic acids research},
  year={1996},
  volume={24 8},
  pages={
          1435-42
        }
}
The hybridization signature approach, using colony filters and labeled complex probes, can provide high throughput measurement of gene activity. We describe here the implementation of this method to follow the expression levels of 47 genes in resting and activated T cells, as well as in epithelial cells. Using 4-fold spotting of colonies, imaging plate detection and various correction and normalization procedures, the technique is sensitive enough to quantify expression levels for sequences… 

Figures and Tables from this paper

Expression scanning of an array of growth control genes in human tumor cell lines

TLDR
The extensive data assembled in this survey identified potential targets of carcinogenesis, for example the CRABP2 and GATA3 transcription factor genes, and the feasibility of this procedure for relatively small tumor samples, without recourse to probe amplification methods.

Applications of nylon membrane arrays to gene expression analysis.

  • J. Cox
  • Biology
    Journal of immunological methods
  • 2001

Global analysis of gene expression in cells of the immune system II. Cell‐free translation products and high‐density filter hybridization data

TLDR
An experimental system for linking information on cell‐free transcription and translation products from cDNA clones with data obtained from hybridization signals from complex probes is developed and used to identify genes involved in thymus development.

Changes in Tumorigenesis- and Angiogenesis-related Gene Transcript Abundance Profiles in Ovarian Cancer Detected by Tailored High Density cDNA Arrays

TLDR
In-house production of tailored cDNA arrays, coupled to comprehensive analysis of resulting hybridization profiles, provides an accessible, reliable, and highly effective method of applying array technology to study disease processes.

Application of Genome-Wide Gene Expression Profiling by High-Density DNA Arrays to the Treatment and Study of Inflammatory Bowel Disease

TLDR
The principles and methods behind DNA array technology, data analysis and mining, and potential application to research and treatment of IBD are detailed.

Profiling expression patterns and isolating differentially expressed genes by cDNA microarray system with colorimetry detection.

TLDR
A high-density cDNA microarray with colorimetry detection system to simultaneously monitor the expression of many genes on nylon membrane and to isolate differentially expressed genes among thousands of genes is described and characterized.

Sensitivity issues in DNA array-based expression measurements and performance of nylon microarrays for small samples.

TLDR
It is demonstrated that the combination of nylon microarrays with(33)P-labelled radioactive probes provides 100-fold better sensitivity, making it possible to perform expression profiling experiments using submicrogram amounts of unamplified total RNA from small biological samples.

From Library Screening to Microarray Technology: Strategies to Determine Gene Expression Profiles and to Identify Differentially Regulated Genes in Plants.

TLDR
In this review, commonly used differential display systems are evaluated and compared and to what extent these techniques have contributed to the authors' understanding of plant gene expression.

Development of high-density DNA microarray membrane for profiling smoke- and hydrogen peroxide-induced genes in a human bronchial epithelial cell line.

  • K. YonedaK. Peck R. Wu
  • Biology
    American journal of respiratory and critical care medicine
  • 2001
TLDR
A complex gene expression array by bronchial epithelial cells in response to the insult of oxidants that are relevant to environmental pollutants is demonstrated.
...

References

SHOWING 1-10 OF 27 REFERENCES

Molecular Cloning: A Laboratory Manual

TLDR
The content has been entirely recast to include nucleic-acid based methods selected as the most widely used and valuable in molecular and cellular biology laboratories.

[Oncogene].

TLDR
Recently, many cellular oncogenes were identified in human cancerous cells by the DNA transfection method, some of which were found to contain sequences related with already known viral oncogenees.

Thymus*

The thymus gland arises as a paired structure from the entoderm of the third and fourth branchial clefts, the anlagen from the fourth cleft, however, remaining rudimentary in most animals. The lobes

Genomics

Nature Genet

  • Nature Genet
  • 1993

Nature Genet

  • Nature Genet
  • 1993

Adv. Immunol

  • Adv. Immunol
  • 1987

Nature Genet

  • Nature Genet
  • 1993

Eur. J. Immunol

  • Eur. J. Immunol
  • 1986

Hum. Mol. Genet

  • Hum. Mol. Genet
  • 1993