Multiplex genome engineering using CRISPR/Cas systems.

@article{Cng2013MultiplexGE,
  title={Multiplex genome engineering using CRISPR/Cas systems.},
  author={L{\^e} Ch{\'i} C{\^o}ng and Fei Ann Ran and David Cox and Shuailiang Lin and Robert P. J. Barretto and Naomi Habib and Patrick David Hsu and Xuebing Wu and Wenyan Jiang and Luciano A Marraffini and Feng Zhang},
  journal={Science},
  year={2013},
  volume={339 6121},
  pages={
          819-23
        }
}
Functional elucidation of causal genetic variants and elements requires precise genome editing technologies. The type II prokaryotic CRISPR (clustered regularly interspaced short palindromic repeats)/Cas adaptive immune system has been shown to facilitate RNA-guided site-specific DNA cleavage. We engineered two different type II CRISPR/Cas systems and demonstrate that Cas9 nucleases can be directed by short RNAs to induce precise cleavage at endogenous genomic loci in human and mouse cells… CONTINUE READING

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