Multiplex fluorogenic real-time PCR for detection and quantification of Escherichia coli O157:H7 in dairy wastewater wetlands.

@article{Ibekwe2002MultiplexFR,
  title={Multiplex fluorogenic real-time PCR for detection and quantification of Escherichia coli O157:H7 in dairy wastewater wetlands.},
  author={Abasiofiok Mark Ibekwe and Pamela M Watt and Catherine M. Grieve and Vijay K. Sharma and Steven R Lyons},
  journal={Applied and environmental microbiology},
  year={2002},
  volume={68 10},
  pages={4853-62}
}
Surface water and groundwater are continuously used as sources of drinking water in many metropolitan areas of the United States. The quality of water from these sources may be reduced due to increases in contaminants such as Escherichia coli from urban and agricultural runoffs. In this study, a multiplex fluorogenic PCR assay was used to quantify E. coli O157:H7 in soil, manure, cow and calf feces, and dairy wastewater in an artificial wetland. Primers and probes were designed to amplify and… CONTINUE READING
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References

Publications referenced by this paper.
Showing 1-10 of 33 references

Detection and quantification of methyl tert-butyl ether-degrading strain PM1 by real-time TaqMan PCR

K. R. Hristova, C. M. Luteneggerand, K. M. Scow
Appl. Environ. Microbiol • 2001

Rapid detection of enterohemorrhagic Escherichia coli by real-time PCR with fluorescent hybridization probes

T. Bellin, M. Pulz, A. Matusset, H.-G. Hempen, F. Gunzer
J. Clin. Microbiol • 2001

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