Multiplex detection of Solenopsis invicta viruses -1, -2, and -3.

  title={Multiplex detection of Solenopsis invicta viruses -1, -2, and -3.},
  author={Steven M Valles and Laura Varone and Leonardo Ramirez and Juan a. Briano},
  journal={Journal of virological methods},
  volume={162 1-2},
Multiplex reverse transcription and polymerase chain reaction (PCR) methods were developed to detect Solenopsis invicta viruses -1, -2, and -3 simultaneously in their host, the red imported fire ant, S. invicta. cDNA synthesis was conducted in a single reaction containing an oligonucleotide primer specific for each virus. Multiplex PCR was subsequently conducted with oligonucleotide primer pairs specific for each virus. The method was specific and sensitive, capable of detecting as few as 500… CONTINUE READING
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