Multiplex conditional mutagenesis in zebrafish using the CRISPR/Cas system.

Abstract

The clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated protein (Cas) system is a powerful tool for genome editing in numerous organisms. However, the system is typically used for gene editing throughout the entire organism. Tissue and temporal specific mutagenesis is often desirable to determine gene function in a specific… (More)
DOI: 10.1016/bs.mcb.2016.04.018

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