Multiplex amplified product‐length polymorphism analysis of 36 mitochondrial single‐nucleotide polymorphisms for haplogrouping of East Asian populations

@article{Umetsu2005MultiplexAP,
  title={Multiplex amplified product‐length polymorphism analysis of 36 mitochondrial single‐nucleotide polymorphisms for haplogrouping of East Asian populations},
  author={K. Umetsu and M. Tanaka and I. Yuasa and N. Adachi and A. Miyoshi and S. Kashimura and Kyung Sook Park and Y. Wei and G. Watanabe and M. Osawa},
  journal={ELECTROPHORESIS},
  year={2005},
  volume={26}
}
  • K. Umetsu, M. Tanaka, +7 authors M. Osawa
  • Published 2005
  • Biology, Medicine
  • ELECTROPHORESIS
  • We present a reliable, rapid, and economical multiplex amplified product‐length polymorphism (APLP) method for analyzing the haplogroup‐diagnostic mitochondrial single‐nucleotide polymorphisms (mtSNPs) in East Asian populations. By examining only 36 haplogroup‐specific mtSNPs in the coding region by using four 9‐multiplex polymerase chain reaction (PCR) and subsequent electrophoresis, we could safely assign 1815 individuals from 8 populations of Japanese, Korean, Chinese, and Germans to 45… CONTINUE READING
    75 Citations

    Topics from this paper

    A New Strategy for the Discrimination of Mitochondrial DNA Haplogroups in Han Population
    • 6
    Multiplex APLP System for High-Resolution Haplogrouping of Extremely Degraded East-Asian Mitochondrial DNAs
    • 8
    • PDF
    Coding region mitochondrial DNA SNPs: targeting East Asian and Native American haplogroups.
    • 98
    • Highly Influenced
    Typing of 24 mtDNA SNPs in a Chinese population using SNaPshot minisequencing
    • Daixin Huang, C. Gui, S. Yi, Qingen Yang, Rongzhi Yang, K. Mei
    • Biology, Medicine
    • Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban
    • 2010
    • 4
    A one step multiplex PCR assay for rapid screening of East Asian mtDNA haplogroups on forensic samples.
    • 3
    Analysis of Y chromosome haplogroups in Japanese population using short amplicons and its application in forensic analysis.
    • 2

    References

    SHOWING 1-10 OF 19 REFERENCES
    Phylogeny of east Asian mitochondrial DNA lineages inferred from complete sequences.
    • 307
    • PDF
    Phylogeographic differentiation of mitochondrial DNA in Han Chinese.
    • 503
    • PDF
    Phylogenetic network for European mtDNA.
    • 356
    • PDF
    Mitochondrial genome variation in eastern Asia and the peopling of Japan.
    • 480
    • PDF