Multiplex amplification of large sets of human exons

  title={Multiplex amplification of large sets of human exons},
  author={Gregory J. Porreca and Kun Qiu Zhang and Jin Billy Li and Bin Xie and Derek W. Austin and Sara L Vassallo and Emily M. LeProust and Bill J. Peck and Christopher Joseph Emig and Fredrik Dahl and Yuan Gao and George M. Church and Jay Shendure},
  journal={Nature Methods},
A new generation of technologies is poised to reduce DNA sequencing costs by several orders of magnitude. But our ability to fully leverage the power of these technologies is crippled by the absence of suitable 'front-end' methods for isolating complex subsets of a mammalian genome at a scale that matches the throughput at which these platforms will routinely operate. We show that targeting oligonucleotides released from programmable microarrays can be used to capture and amplify ∼10,000 human… CONTINUE READING
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