Multiplex PCR method for identifying recombinant vaccine-related polioviruses.

Abstract

The recent discovery of recombinant circulating vaccine-derived poliovirus (recombinant cVDPV) has highlighted the need for enhanced global poliovirus surveillance to assure timely detection of any future cVDPV outbreaks. Six pairs of Sabin strain-specific recombinant primers were designed to permit rapid screening for VDPV recombinants by PCR.

Cite this paper

@article{Kilpatrick2004MultiplexPM, title={Multiplex PCR method for identifying recombinant vaccine-related polioviruses.}, author={David R. Kilpatrick and Karen Z. Ching and Jane Iber and Ray Campagnoli and Christopher J. Freeman and Nada G Mishrik and Hongmei Liu and Mark A. Pallansch and Olen Kew}, journal={Journal of clinical microbiology}, year={2004}, volume={42 9}, pages={4313-5} }