Multiplex PCR method for detection of three Aeromonas enterotoxin genes.

@article{Kingombe2010MultiplexPM,
  title={Multiplex PCR method for detection of three Aeromonas enterotoxin genes.},
  author={Cesar I Bin Kingombe and Jean-Yves D'Aoust and Geert Huys and Lisa Hofmann and Mary Rao and Judy Kwan},
  journal={Applied and environmental microbiology},
  year={2010},
  volume={76 2},
  pages={425-33}
}
A novel multiplex PCR method using three sets of specific primers was developed for the detection of the cytotoxic (act), heat-labile (alt), and heat-stable (ast) enterotoxin genes in Aeromonas spp. This assay was used to characterize 35 reference strains as well as 537 food-borne isolates. A total of seven gene pattern combinations were encountered, including act, alt, act/alt, act/alt/ast, act/alt/148-bp amplicon, alt/ast, and alt/148-bp amplicon. The alt gene was detected with 34 reference… CONTINUE READING

Citations

Publications citing this paper.
Showing 1-10 of 13 extracted citations

References

Publications referenced by this paper.
Showing 1-10 of 24 references

Similar Papers

Loading similar papers…