Multiplex PCR for the detection of tetracycline resistant genes.

@article{Ng2001MultiplexPF,
  title={Multiplex PCR for the detection of tetracycline resistant genes.},
  author={Lai Kuen April Ng and Irene Luque Mart{\'i}n and Michelle Alfa and Michael S. Mulvey},
  journal={Molecular and cellular probes},
  year={2001},
  volume={15 4},
  pages={
          209-15
        }
}
Specific primer pairs were selected for the PCR amplification of 14 tetracycline resistant genes commonly found in Gram positive and Gram negative organisms. Combinations of primer pairs were used in multiplex PCR reactions to detect specific groups of tet genes as follows; Group I tet (B), tet (C), tet (D); Group II tet (A), tet (E), tet (G); Group III tet (K), tet (L), tet (M), tet (O), tet (S); Group IV tetA (P), tet (Q), tet (X). To test the multiplex PCR, Groups I and II were used on 25… CONTINUE READING
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