Multiplex PCR for the detection of Brucella ovis, Actinobacillus seminis and Histophilus somni in ram semen.

@article{Saunders2007MultiplexPF,
  title={Multiplex PCR for the detection of Brucella ovis, Actinobacillus seminis and Histophilus somni in ram semen.},
  author={Vanessa F Saunders and Leslie A Reddacliff and Trygve Berg and Michael A. Hornitzky},
  journal={Australian veterinary journal},
  year={2007},
  volume={85 1-2},
  pages={72-7; quiz 85}
}
OBJECTIVE To develop a multiplex polymerase chain reaction (PCR) assay for the rapid detection of Brucella ovis, Actinobacillus seminis, Histophilus somni in fresh ram semen samples. DESIGN The multiplex assay was based on the single PCR assays published for the detection of A seminis and B ovis, and the forward primer published for the detection of H somni; an alternative reverse primer for H somni was designed in this study. PROCEDURE Culture and PCR of 295 fresh semen samples were… CONTINUE READING

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