Multiplex PCR for the detection and quantification of zoonotic taxa of Giardia, Cryptosporidium and Toxoplasma in wastewater and mussels.

@article{Marangi2015MultiplexPF,
  title={Multiplex PCR for the detection and quantification of zoonotic taxa of Giardia, Cryptosporidium and Toxoplasma in wastewater and mussels.},
  author={Marianna Marangi and Annunziata Giangaspero and Vita Lacasella and A. Lonigro and Robin B. Gasser},
  journal={Molecular and cellular probes},
  year={2015},
  volume={29 2},
  pages={
          122-5
        }
}
Giardia duodenalis, Cryptosporidium parvum and Toxoplasma gondii are important parasitic protists linked to water- and food-borne diseases. The accurate detection of these pathogens is central to the diagnosis, tracking, monitoring and surveillance of these protists in humans, animals and the environment. In this study, we established a multiplex real-time PCR (qPCR), coupled to high resolution melting (HRM) analysis, for the specific detection and quantification of each G. duodenalis… CONTINUE READING
2
Twitter Mentions