Multiplex PCR for detection of trait and virulence factors in enterohemorrhagic Escherichia coli serotypes.

Abstract

A multiplex PCR assay was developed which allowed the simultaneous detection of five trait genes or virulence markers in enterohemorrhagic Escherichia coli (EHEC) serotypes. A primer pair, designed to detect a single base-pair mutation in the uidA gene, is specific only for the prototypic EHEC of O157:H7 serotype and its toxigenic, non-motile variants. In a… (More)

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Cite this paper

@article{Feng2000MultiplexPF, title={Multiplex PCR for detection of trait and virulence factors in enterohemorrhagic Escherichia coli serotypes.}, author={Pingyun Feng and Steven R. Monday}, journal={Molecular and cellular probes}, year={2000}, volume={14 6}, pages={333-7} }