Multiplex PCR for detection of stx genes of Escherichia coli.

  • Habibur Rahman
  • Published 2002 in The Indian journal of medical research

Abstract

BACKGROUND & OBJECTIVE Shiga toxin producing Escherichia coli (STEC), which includes both enterohaemorrhagic Esch. coli (EHEC, 0157: 7H) and non-EHEC (non-0157) produces two major Shiga toxins (Stx1 & Stx2). Detection of Stx or stx genes is the only approach to detect all the different types of STEC. A multiplex PCR is used for the detection of stx genes… (More)

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