Multiplex PCR-based reverse line blot hybridization assay to identify 23 Streptococcus pneumoniae polysaccharide vaccine serotypes.

@article{Kong2006MultiplexPR,
  title={Multiplex PCR-based reverse line blot hybridization assay to identify 23 Streptococcus pneumoniae polysaccharide vaccine serotypes.},
  author={Fanrong Kong and Mitchell Brown and Archcna Sabananthan and Xianyu Zeng and Gwendolyn L. Gilbert},
  journal={Journal of clinical microbiology},
  year={2006},
  volume={44 5},
  pages={1887-91}
}
We developed a multiplex PCR-based reverse line blot assay to identify 23 pneumococcal serotypes represented in the polysaccharide vaccine, using 334 well-characterized isolates, representing all 90 serotypes, and 268 "unknowns." The assay identified all target serotypes, but 11, which cross-react with 1 to 4 nonvaccine serotypes, could be distinguished using serotype-specific antisera.