Multiplex PCR assay for the identification of nivalenol, 3- and 15-acetyl-deoxynivalenol chemotypes in Fusarium.

@article{Quarta2006MultiplexPA,
  title={Multiplex PCR assay for the identification of nivalenol, 3- and 15-acetyl-deoxynivalenol chemotypes in Fusarium.},
  author={Angela Quarta and Giovanni Mita and Miriam Edith Haidukowski and Antonio Francesco Logrieco and Giuseppina Mul{\`e} and Angelo Visconti},
  journal={FEMS microbiology letters},
  year={2006},
  volume={259 1},
  pages={7-13}
}
The ability to rapidly distinguish trichothecene chemotypes in a given species/population of the genus Fusarium is important due to significant differences in the toxicity of these secondary metabolites. A multiplex PCR assay, based on primer pairs derived from the Tri3, Tri5 and Tri7 genes of the trichothecene gene cluster was established for the identification of the different chemotypes among Fusarium graminearum, F. culmorum and F. cerealis. Using the selected primers, specific… CONTINUE READING

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