Multiplex PCR assay for detection of recombinant genes encoding fatty acid desaturases fused with lichenase reporter protein in GM plants.

@article{Berdichevets2010MultiplexPA,
  title={Multiplex PCR assay for detection of recombinant genes encoding fatty acid desaturases fused with lichenase reporter protein in GM plants.},
  author={Iryna N Berdichevets and Hristina R Shimshilashvili and Iryna M Gerasymenko and Yana R Sindarovska and Yuriy V Sheludko and Irina V. Goldenkova-Pavlova},
  journal={Analytical and bioanalytical chemistry},
  year={2010},
  volume={397 6},
  pages={2289-93}
}
Thermostable lichenase encoded by licB gene of Clostridium thermocellum can be used as a reporter protein in plant, bacterial, yeast, and mammalian cells. It has important advantages of high sensitivity and specificity in qualitative and quantitative assays. Deletion variants of LicB (e.g., LicBM3) retain its enzymatic activity and thermostability and can… CONTINUE READING