Multiplex PCR assay and phylogenetic analysis of sequences derived from D2 domain of 28S rDNA distinguished members of the Anopheles culicifacies complex into two groups, A/D and B/C/E.

@article{Raghavendra2009MultiplexPA,
  title={Multiplex PCR assay and phylogenetic analysis of sequences derived from D2 domain of 28S rDNA distinguished members of the Anopheles culicifacies complex into two groups, A/D and B/C/E.},
  author={Kamaraju Raghavendra and Anthony John Cornel and B. P. Niranjan Reddy and Frank H. Collins and Narasinga Prasad Nanda and Dinesh Chandra and Vaishali Verma and Aditya Dash and S. K. Subbarao},
  journal={Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases},
  year={2009},
  volume={9 2},
  pages={
          271-7
        }
}
A multiplex PCR assay was developed using the sequences of the D2 region of 28S ribosomal DNA (rDNA) to discriminate the five members of the Anopheles culicifacies complex provisionally designated as species A, B, C, D and E. Two minus strand primers derived from sequence differences in the D2 variable region and a universal plus strand primer derived from the conserved 28S (rDNA) has delimited five members into species A and D (group 1) and species B, C and E (group 2) in a PCR diagnostic… CONTINUE READING
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