Multiplex PCR: use of heat-stable Thermus thermophilus RecA protein to minimize non-specific PCR products

@article{Shigemori2005MultiplexPU,
  title={Multiplex PCR: use of heat-stable Thermus thermophilus RecA protein to minimize non-specific PCR products},
  author={Yasushi Shigemori and Tsutomu Mikawa and Takehiko Wako-shi Shibata and Michio Oishi},
  journal={Nucleic Acids Research},
  year={2005},
  volume={33},
  pages={e126 - e126}
}
In this paper we report that the inclusion of heat-resistant RecA protein from a thermophilic bacteria, Thermus thermophilus, and its cofactor (ATP) in PCR effectively eliminates non-specific PCR products. The effect of RecA protein, which catalyzes pairing between homologous DNA molecules with great fidelity in genetic recombination, is due to its promotion of precise priming in PCR (i.e. priming at sites where the primer sequence is completely complementary to that of the target sequence). In… CONTINUE READING

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