Multiplex PCR: optimization and application in diagnostic virology.

  title={Multiplex PCR: optimization and application in diagnostic virology.},
  author={Elfateh M Elnifro and Ahmed M Ashshi and Robert J. Cooper and Paul E. Klapper},
  journal={Clinical microbiology reviews},
  volume={13 4},
PCR has revolutionized the field of infectious disease diagnosis. To overcome the inherent disadvantage of cost and to improve the diagnostic capacity of the test, multiplex PCR, a variant of the test in which more than one target sequence is amplified using more than one pair of primers, has been developed. Multiplex PCRs to detect viral, bacterial, and/or other infectious agents in one reaction tube have been described. Early studies highlighted the obstacles that can jeopardize the… CONTINUE READING