Multiplex PCR/liquid chromatography assay for detection of gene rearrangements: application to RB1 gene.

@article{Dehainault2004MultiplexPC,
  title={Multiplex PCR/liquid chromatography assay for detection of gene rearrangements: application to RB1 gene.},
  author={Catherine Dehainault and Anthony Laug{\'e} and Virginie Caux-Moncoutier and Sabine Pag{\`e}s-Berhouet and Jordan Berlin and Laurence Desjardins and J{\'e}r{\^o}me Couturier and L. Brugieres and Dominique Stoppa-Lyonnet and Claude Houdayer},
  journal={Nucleic acids research},
  year={2004},
  volume={32 18},
  pages={
          e139
        }
}
Screening for large gene rearrangements is established as an important part of molecular medicine but is also challenging. A variety of robust methods can detect whole-gene deletions, but will fail to detect more subtle rearrangements that may involve a single exon. In this paper, we describe a new, versatile and robust method to assess exon copy number, called multiplex PCR/liquid chromatography assay (MP/LC). Multiple exons are amplified using unlabeled primers, then separated by ion-pair… CONTINUE READING

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