Multiplex CRISPR/Cas9-based genome engineering from a single lentiviral vector

@inproceedings{Kabadi2014MultiplexCG,
  title={Multiplex CRISPR/Cas9-based genome engineering from a single lentiviral vector},
  author={Ami M. Kabadi and David G. Ousterout and Isaac B Hilton and Charles A Gersbach},
  booktitle={Nucleic acids research},
  year={2014}
}
Engineered DNA-binding proteins that manipulate the human genome and transcriptome have enabled rapid advances in biomedical research. In particular, the RNA-guided CRISPR/Cas9 system has recently been engineered to create site-specific double-strand breaks for genome editing or to direct targeted transcriptional regulation. A unique capability of the… CONTINUE READING