Multiple transcription start sites of the carrot dihydrofolate reductase-thymidylate synthase gene, and sub-cellular localization of the bifunctional protein

@article{Luo1997MultipleTS,
  title={Multiple transcription start sites of the carrot dihydrofolate reductase-thymidylate synthase gene, and sub-cellular localization of the bifunctional protein},
  author={Meizhong Luo and Roberta Orsi and Emanuela Patrucco and Simonetta Pancaldi and Rino Cella},
  journal={Plant Molecular Biology},
  year={1997},
  volume={33},
  pages={709-722}
}
The analysis of clones obtained by rapid amplification of the 5′ endand by primer extension of the mRNA for carrot bifunctionaldihydrofolate reductase-thymidylate synthase showed transcripts ofdiffering lengths that belonged to two sub-populations. The longertranscripts were found to contain a translation start site 147 ntupstream of, and in frame with, the one which is present in the shortertranscripts. The ORF that begins at this ATG codes for a protein of64714 Da, which is much larger than… CONTINUE READING