Multiple-step ligand injection affinity capillary electrophoresis for determining binding constants of ligands to receptors.

@article{Zhang2000MultiplestepLI,
  title={Multiple-step ligand injection affinity capillary electrophoresis for determining binding constants of ligands to receptors.},
  author={Yan Jing Zhang and Frank A. Gomez},
  journal={Journal of chromatography. A},
  year={2000},
  volume={897 1-2},
  pages={
          339-47
        }
}
This work demonstrates the use of multiple-step ligand injection affinity capillary electrophoresis (ACE) using two model systems: vancomycin from Streptomyces orientalis and carbonic anhydrase B (CAB, EC 4.2.1.1). In this technique a sample plug of receptor and non-interacting standards is injected by pressure and electrophoresed in a buffer containing a given concentration of ligand. The sequence is repeated for all concentrations of ligand generating a single electropherogram containing a… CONTINUE READING
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