Multiple species of mammalian S-adenosylmethionine synthetase. Partial purification and characterization.

@article{Okada1981MultipleSO,
  title={Multiple species of mammalian S-adenosylmethionine synthetase. Partial purification and characterization.},
  author={Gen Okada and Hirobumi Teraoka and Kazuhiro Tsukada},
  journal={Biochemistry},
  year={1981},
  volume={20 4},
  pages={934-40}
}
Two species of S-adenosylmethionine (S-Ado-Met) synthetase (EC 2.5.1.6) exist in rat liver cytosol and a distinct species of the enzyme exists in kidney cytosol. S-Ado-Met synthetases alpha and beta in rat liver cytosol have been partially purified about 200- and 80-fold, respectively. The apparent molecular weight estimated by gel filtration and the sedimentation coefficient are 210 000 and 9 S for S-Ado-Met synthetase alpha and 160 000 and 5.5 S for S-Ado Met synthetase beta. Both enzymes… CONTINUE READING