Multiple segmental and selective isotope labeling of large RNA for NMR structural studies

Abstract

Multiple segmental and selective isotope labeling of RNA with three segments has been demonstrated by introducing an RNA segment, selectively labeled with (13)C(9)/(15)N(2)/(2)H((1', 3', 4', 5', 5''))-labeled uridine residues, into the central position of the 20 kDa epsilon-RNA of Duck Hepatitis B Virus. The RNA molecules were produced via two efficient… (More)
DOI: 10.1093/nar/gkn397

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@inproceedings{Nelissen2008MultipleSA, title={Multiple segmental and selective isotope labeling of large RNA for NMR structural studies}, author={Frank H. T. Nelissen and Adriaan J. van Gammeren and Marco Tessari and Frederic C. Girard and Hans A. Heus and Sybren S. Wijmenga}, booktitle={Nucleic acids research}, year={2008} }