Multiple roles for the C-terminal domain of eIF5 in translation initiation complex assembly and GTPase activation.

@article{Asano2001MultipleRF,
  title={Multiple roles for the C-terminal domain of eIF5 in translation initiation complex assembly and GTPase activation.},
  author={Katsura Asano and Anath Shalev and Lon Phan and Kathy S Nielsen and J. Clayton and Leo{\vs} Shivaya Val{\'a}{\vs}ek and Thomas F. Donahue and Alan G Hinnebusch},
  journal={The EMBO journal},
  year={2001},
  volume={20 9},
  pages={2326-37}
}
eIF5 stimulates the GTPase activity of eIF2 bound to Met-tRNA(i)(Met), and its C-terminal domain (eIF5-CTD) bridges interaction between eIF2 and eIF3/eIF1 in a multifactor complex containing Met-tRNA(i)(Met). The tif5-7A mutation in eIF5-CTD, which destabilizes the multifactor complex in vivo, reduced the binding of Met-tRNA(i)(Met) and mRNA to 40S subunits in vitro. Interestingly, eIF5-CTD bound simultaneously to the eIF4G subunit of the cap-binding complex and the NIP1 subunit of eIF3. These… CONTINUE READING