Multiple pulse NMR


This report is a continuation of the review from last year and surveys literature on multiple resonance methods published in the period June 2005 to May 2006. The current review period featured a remarkable number of contributions that accelerate the recording of multidimensional data. New acquisition schemes emerged that exploit the benefits of faster magnetization recovery and allow faster pulsing thus increasing sensitivity per unit time. Further improvements were described using limited sampling and processing methods which can lead to time-savings of one order of magnitude compared to conventional sampling schemes. These novel acquisition and processing techniques can be combined to bring even larger savings in time. Accordingly a wealth of new or modified triple-resonance assignment experiments has emerged. Further, an amino acid selective position-specific protonation approach has been introduced for efficient and improved structure determination. Protonless methods have been improved for the study of paramagnetic proteins. Additional developments were reported in the areas of side chain and backbone relaxation measurements in particular with applications to off-resonance spinlock and/or relaxation dispersion experiments to measure exchange contributions or in the use of MQ relaxation to monitor correlated protein motions. Continuing improvements were reported for scalar coupling and RDC measurements.

Cite this paper

@inproceedings{Nietlispach2007MultiplePN, title={Multiple pulse NMR}, author={Daniel Nietlispach}, year={2007} }