Multiple primer pairs for polymerase chain reaction (PCR) amplification of human parvovirus B19 DNA.

@article{Durigon1993MultiplePP,
  title={Multiple primer pairs for polymerase chain reaction (PCR) amplification of human parvovirus B19 DNA.},
  author={Edison Luiz Durigon and Dean D Erdman and G{\'e}rard Gary and Mark A. Pallansch and Thomas J Torok and Larry J. Anderson},
  journal={Journal of virological methods},
  year={1993},
  volume={44 2-3},
  pages={155-65}
}
Human parvovirus B19 is the etiologic agent of erythema infectiosum and transient aplastic crisis in patients with hemolytic anemias and has been associated with fetal death, arthritis, and chronic anemia. Acute B19 infection is best diagnosed by detection of IgM antibodies, whereas the diagnosis of chronic infection often requires the sensitivity of PCR to demonstrate presence of virus over time. To improve our ability to detect B19 DNA by polymerase chain reaction (PCR), we evaluated 19… CONTINUE READING
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