Multiple pathways of N-kinase activation in PC12 cells.

  title={Multiple pathways of N-kinase activation in PC12 cells.},
  author={E Rowland-Gagn{\'e} and Lloyd A Greene},
  journal={Journal of neurochemistry},
  volume={54 2},
Past work established a cell-free assay for a nerve growth factor (NGF)-activated protein kinase activity (designated N-kinase) that utilizes tyrosine hydroxylase and histone H1 as substrates and that is distinct from a variety of well-characterized kinases. This study explores the specificity and mechanistic pathway(s) by which N-kinase activity is regulated in PC12 rat pheochromocytoma cells. N-kinase is rapidly activated in these cells by treatment with NGF, epidermal growth factor (EGF… CONTINUE READING