Multiple nonidentical reductive-dehalogenase-homologous genes are common in Dehalococcoides.

@article{Hlscher2004MultipleNR,
  title={Multiple nonidentical reductive-dehalogenase-homologous genes are common in Dehalococcoides.},
  author={Tina H{\"o}lscher and Rosa Krajmalnik-Brown and Kirsti M. Ritalahti and Friedrich von Wintzingerode and Helmut Goerisch and Frank E L{\"o}ffler and Lorenz Adrian},
  journal={Applied and environmental microbiology},
  year={2004},
  volume={70 9},
  pages={
          5290-7
        }
}
Degenerate primers were used to amplify large fragments of reductive-dehalogenase-homologous (RDH) genes from genomic DNA of two Dehalococcoides populations, the chlorobenzene- and dioxin-dechlorinating strain CBDB1 and the trichloroethene-dechlorinating strain FL2. The amplicons (1,350 to 1,495 bp) corresponded to nearly complete open reading frames of known reductive dehalogenase genes and short fragments (approximately 90 bp) of genes encoding putative membrane-anchoring proteins. Cloning… CONTINUE READING
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