Multiple new phenotypes induced in 10T 1 2 and 3T3 cells treated with 5-azacytidine

  title={Multiple new phenotypes induced in 10T
 and 3T3 cells treated with 5-azacytidine},
  author={Shirley M. Taylor and Peter A. Jones},

Changes in hematopoiesis-supporting ability of C3H10T1/2 mouse embryo fibroblasts during differentiation.

Northern analysis showed that the preadipocyte cell line acquired the ability to produce a significant amount of stem cell factor (SCF), interleukin-6 (IL-6), leukemia inhibitory factor, and macrophage colony-stimulating factor mRNAs in response to IL-1 or lipopolysaccharide stimulation.

Changes in phenotypic expression in embryonic and adult cells treated with 5‐azacytidine

5‐azacytidine induced the formation of biochemically differentiated myotubes, adipocytes, and chondrocytes in the mouse embryo cell line and the induction of the muscle phenotype was cell cycle specific, while the adipocyte phenotype is also induced maximally in cells treated during early S phase.

Effect of cellular determination on oncogenic transformation by chemicals and oncogenes

The presence of as many as six copies of the c-Ha-ras gene per genome did not prevent the formation of striated muscle cells which expressed immunologically detectable muscle-specific myosin and the expression of the determination gene for myogenesis or prevent end-stage myogenic differentiation.

Growth hormone facilitates 5'-azacytidine-induced myogenic but inhibits 5'-azacytidine-induced adipogenic commitment in C3H10T1/2 mesenchymal stem cells.

  • D. JiaWeijiang ZhengHonglin Jiang
  • Biology
    Growth hormone & IGF research : official journal of the Growth Hormone Research Society and the International IGF Research Society
  • 2018

Promoting effect of 5-azacytidine on the myogenic differentiation of bone marrow stromal cells.

Bone morphogenetic protein-2 causes commitment and differentiation in C3H10T1/2 and 3T3 cells.

The results in this model system indicate that a single protein factor can cause differentiation of a stem cell line to multiple phenotypes, that phenotypes induced can be regulated by factor concentration, and that other factors can also influence BMP-2 induced differentiation.

5-Azacytidine induces changes in electrophysiological properties of human mesenchymal stem cells

The results suggest that despite the absence of differentiation of hMSC into cardiomyocytes, treatment with 5-azacytidine caused profound changes in current density.



Functional striated muscle cells from non-myoblast precursors following 5-azacytidine treatment

Experiments are described showing that the multinucleated tubular cells which arise in C3H 10T½ C18 cells treated with aza-CR are in fact functional myotubes.

In vitro correlates of transformation in C3H/10T1/2 clone 8 mouse cells.

All transformed cells showed an increase in extracellular fibrinolytic activity which may be an additional marker for transformation, and the degree of tumorigenicity was best predicted by the relative plating efficiencies of the morphologically transformed cells in soft agar.

Establishment and characterization of a cloned line of C3H mouse embryo cells sensitive to postconfluence inhibition of division.

No spontaneous transformation in vitro has been observed in the stock cultures transferred on a regular schedule and tests for tumorigenicity at all passages were negative.

Inhibition of adipose conversion of 3T3 fibroblasts by tumour promoters

Another cell system, mouse preadipose cells, is described, in which tumour promoters inhibit terminal differentiation, and it is confirmed that there is a good correlation between the ability of a particular phorbol diester to promote tumours in mouse skin and its ability to inhibit terminals differentiation of cells in culture.

Induction of morphological transformation in mouse C3H/10T1/2 clone 8 cells and chromosomal damage in hamster A(T1)C1-3 cells by cancer chemotherapeutic agents.

Various cancer chemotherapeutic agents including alkylating agents, antimetabolites, and antibiotics or natural products were studied for their ability to produce morphological transformation in the

Induction of lipogenesis during differentiation in a "preadipocyte" cell line.

3T3-L1 fibroblasts differentiate in culture into cells having adipocyte character and the elevated acetyl-CoA carboxylase activity is the product of an increased cellular enzyme level.